Intact molecular weight determination by MALDI-TOFMS

PharmaCadence can now offer intact molecular weight measurements of proteins and peptides by MALDI TOFMS. The use of surrogate peptides for the quantification of proteins has become standard practice. However, there are many potential problems when using a peptide to represent an entire protein. By applying MALDI TOFMS in the early stages of method development for surrogate peptide assays, better information can be obtained about the nature of the protein being analyzed. MALDI TOFMS molecular weight determinations have also proved valuable for the generation of active immunogens for antibody preparation. Knowing the number and distribution of haptens conjugated can help quickly identify the best approach to antigen synthesis.