The team at PharmaCadence has taken on the challenge of defining quantitative analysis with LC-MS in the spirit of systems biology. The group has demonstrated methods that are capable of quantifying both large and small molecule components of an enzyme complex to assist biological researchers in their quest to study and understand biological systems rather than just the individual molecular components making up those systems. Dr. Rick King, Laboratory Director at PharmaCadence recently presented the approach at the CPSA 2012 conference. He showed data from UGT enzyme systems where a single method was used to quantify the UGT 2B7 enzyme, the AZT drug substrate and the AZT-glucuronide conjugate formed as a product of the reaction providing a more complete biological picture from a single analysis method.
